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ispA [2018-09-13 18:10:59]
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ispA [2018-09-13 18:10:59]

intracellular serine protease
locus
BSU13190
pI
4.52
mw
33.69 kDa
protein length
319 aa Sequence Blast
gene length
957 bp Sequence Blast
function
protein degradation
product
intracellular serine protease
essential
no
synonyms
ispI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]

    • Gene

    Coordinates
    1,386,024 → 1,386,983

    The protein

    Protein family

  • peptidase S8 family (according to Swiss-Prot)

    • Structure

  • [PDB|2WV7] (from Bacillus clausii, 53% identity) [pubmed|20541512]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|53364B1E6728EA385E95F96E47AFDE6D65AD7040|ispA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3087947], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|23569278], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|23569278]
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    Biological materials

    Mutant

  • BKE13190 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAGCCCTCCTTTT, downstream forward: _UP4_TAAGATTATTTTTCTTATAT
  • BKK13190 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAGCCCTCCTTTT, downstream forward: _UP4_TAAGATTATTTTTCTTATAT

    • References

  • 3087947,23569278,20541512